Polymeric scaffolds play a central role in bioengineering applications. The mechanical and morphological properties of these biomaterial matrices can be modified by altering their composition and/or synthesis process in order to meet the requirements of certain biological functionalities. Thanks to that scaffolds can be used to mimick the surrounding extracellular environment in which established and primary cell lines can proliferate and differentiate [1, 2, 3, 4].
In our work we show that micro computed tomography (µCT) is a unique methods to investigate the morphology of scaffolds intended for use in different applications: cryogel matrices used to model the prostate gland tissue in which the prostate cancer cells can proliferate  and polycaprolactone (PCL) scaffolds, which are good candidates for the substitution of bone tissues . By complementing the non-destructive structural study of scaffolds architecture performed by SRµT with mechanical tests and measurements of the cellular metabolic activity it was possible to identify the scaffolds, meeting the requirement of the desired application.
The µCT grants 3D visualization also for scaffolds after in vitro and ex vivo experiments, permitting to represent the formation and distribution of multi cellular tumor spheroids cultured inside the optimal cryogel scaffold composition (in vitro) and to visualize the ingrown tissue in the dissected PCL scaffolds (ex vivo).