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Poster

Investigating Dynamic Biological Processes with High-Speed, High-Resolution Correlative AFM-Light Microscopy

Part of:
Line-Up:
- Plenary Lecture Test Ploetz 1 Alexia Ploetz
- Poster Investigating Dynamic Biological Processes with High-Speed, High-Resolution Correlative AFM-Light Microscopy 1 Oilibhe Pabsch
- Poster Corrosion behaviour of potencially biodegradable ternary alloy Zn-Mg-Ca 1 Dipl.-Ing. Jan Pinc
- Poster Multi scale topography in titanium alloys by electron beam technique: influence on the tissue adhesion and bacterial contamination 1 Dr. Fernando Warchomicka
- Poster Cytotoxicity of gold nanorods and nanoribbons in context of biomedical applications 1 Marika Musielak
- Poster Preliminary studies of synthesis of gold nanoribbons using selected dicationic surfactants 1 Joanna Patalas
- Poster Diclofenac release from a silicon based hydrogel for contact lenses controlled by a layer-by-layer coating 1 Prof. Ana Serro
- Poster Synthesis, solubility and optical activity of chiral poly(2,4-disubstituted-2-oxazoline)s 1 Mengshi Yang
- Poster Influence of Cu2+-doping in β-TCP on the hydration kinetics of brushite cement investigated by Rietveld analysis and heat flow calorimetry 1 Karla Späth
- Poster Analytical investigation of the influence of micro- and nanoplastic particles in crop plants by electron microscopic imaging and spectroscopy 1 Dr. Jörg Wissler
- Poster Influence of fluoride corrosion attack on the metal-porcelain bond strength of TiCr20 compared to titanium 1 Christine Schille
- Poster Bacterial nanocellulose loaded with frankincense extract as natural wound dressing to treat local dermal inflammations 1 Berit Karl
- Poster Graded functionalization of polydopamine coated fiber mats as potential implant for tendon-bone-transition 1 Sarah Oehmichen
- Poster Enhancing the functionalities of bioactive glass-based scaffolds by novel polymeric coatings 1 Dr. Liliana Liverani
- Poster Durability analysis of the cryopreservation of DNA origami nanostructures 1 Yang Xin
- Poster Effects of staple age on DNA origami nanostructure assembly and stability 1 Charlotte Kielar
- Poster Dual-setting brushite-silica gel cements – Variations of the monomer precursors and tuning of the setting parameters 1 Ib Holzmeister
- Poster Novel QCM-D/microscopy set-up for real-time analysis of bacterial binding onto biomaterial-coated sensors 1 Dr. Zeqian Xu
- Poster Chairside plasma activation of encapsulated implants: how fast is it? 1 Miguel Angel Garcia Chame
- Poster Nanostructured high-pressure laminate surfaces to reduce transmission pathways of pathogens in public transportation 1 Christian Helbing
- Poster Biomineralization of Recombinant Spider Silk Films 1 Vanessa J. Neubauer
- Poster Modification of bacterial cellulose to enhance anti-inflammatory wound dressing properties 1 Uwe Beekmann
- Poster Absolute Characterization of Synthetic Macromolecules used in Biomaterials Science Applications 1 Mandy Grube
- Poster Biodegradable thermoplastic materials for bimodal imaging containing the mineral present in the human body 1 Zaneta Górecka
- Poster Nano-rough titanium surfaces created by wet alkaline etching and PVD and their influence on bacterial adhesion as well as protein adsorption 1 Michaela Schürmann
- Poster Effective strategies for the controlling and dealing with transmission pathway of pathogens in air traffi 1 Christian Helbing
- Poster Cytotoxicity evaluation of biodegradable Zn-4Cu alloy using L929, Saos-2 and TAg cell lines 1 Ping Li
- Poster Influence of pulsed bias voltage on the tribological and morphological properties of a-C coatings deposited by an anodic arc method 1 Dipl.-Ing. Ingo Erdmann

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The ability of atomic force microscopy (AFM) to obtain three-dimensional topography images of biological molecules and complexes with nanometer resolution and under near-physiological conditions remains unmatched by other imaging techniques. However, the typically longer image acquisition times required to obtain a single high-resolution image (~minutes) has limited the advancement of AFM for investigating dynamic biological processes.

 

While recent years have shown significant progress in the development of high-speed AFM (HS-AFM), the ability to scan faster has typically been achieved at the cost of decreased scanner range and restricted sample size. As such, these HS-AFM systems have mainly been focused on studying single molecule dynamics and have been very limited in their ability to conduct live cell imaging.

 

JPK BioAFM has developed a new NanoWizard® ULTRA Speed 2 AFM which not only enables high-speed studies of time-resolved dynamics associated with cellular processes, it’s latest scanner technologies and compact design also allow full integration of AFM into advanced commercially available light microscopy techniques. Thus, fast AFM imaging of 10 frames per second can be seamlessly combined with methods such as epi-fluorescence, confocal, TIRF, STED microscopy, and many more. Furthermore, with the new NestedScanner technology, cells, bacteria or structured surfaces with sample heights of up to 8 μm can now be examined at the highest scan speeds.

 

We will present how the latest advances in the ULTRA Speed 2 AFM can be applied to study a wide-range of biological samples, from individual biomolecules to mammalian cells and tissues in real-time, in-situ experiments. We will also describe how this unique system enables new research opportunities with high-speed, high-resolution correlative AFM-light microscopy.

 

Speaker:
Oilibhe Pabsch
Bruker Nano GmbH
Additional Authors:
  • Dr. Torsten Mueller
    Bruker Nano GmbH
  • Dr. Heiko Haschke
    Bruker Nano GmbH