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Oral Poster

The PLGA fiber component of brushite-forming calcium phosphate cement induces the osteogenic differentiation of human adipose tissue-derived stem cells

Part of:
18:40 Oral Poster Effect of surface processing on roughness, wettability and cytotoxicity of carbon fiber-reinforced polyetheretherketone (PEEK) composite fabricated by fused deposition modeling 1 Dr. Xingting Han
18:43 Oral Poster Characterisation of bactericidal titanium surfaces using electron microscopy 1 Ph.D. Joshua Jenkins
18:49 Oral Poster Parvovirus B19 virus-like particles as platform for the development of biomaterials. 1 Prof. Dr. Ismael Bustos-Jaimes
18:52 Oral Poster The Influence of Surface Nanopatterns with varying Periodicities on Protein adsorption 1 Yu Yang
18:55 Oral Poster Opaque, Translucent and Transparent Flower Petals 1 Takumi Arakawa
18:58 Oral Poster Impact of Surface Morphology and Net Charge of Spider Silk based Materials on Interactions with Human Blood Components 1 Sarah Lentz
19:01 Oral Poster Modulation of Bacteria on Titanium Surfaces via Nanotopography and Antimicrobial Peptides 1 Marcus Eales
19:04 Oral Poster Degradable hybrid polymers for tissue engineering 1 Johannes Schwaiger
19:07 Oral Poster Antibacterial functionalization of plasma-chemical oxidized titanium surfaces 1 Dr. Stefan Kranz
19:10 Oral Poster Thermal crosslinking of hydrophilic polymers using polyperoxides 1 Ph.D. Solomiia Borova
19:13 Oral Poster Poly(peptoid)s: Peptidomimetics as potential Biomaterials 1 Christine Schlutt
19:16 Oral Poster A Nanoscaled Hybrid Coating for Neuronal Electrodes: Nanoporous Silica NPs Embodied into Nanoporous Platinum. 1 Tim-Joshua Pinkvos
19:19 Oral Poster Drug Delivery from Nanoporous Titania Coatings for Dental Application 1 Saskia Zailskas
19:22 Oral Poster Waste to resource: Biopolymer from industrial wastewater 1 Prof. Regina Nogueira
19:25 Oral Poster Divide and Comprehend: Separating the Effects of Hydrophobicity / Hydrophilicity and Crystallinity on the Thermal Properties of Polyester Based Drug Delivery Systems 1 Karl Scheuer
19:28 Oral Poster Elastic Polymer-Coated Nano- and Macroporous Bioactive Glass-Composites for Bone Regeneration 1 Marvin Lietzow
19:34 Oral Poster Alginate/silk fibroin-based hydrogels for vascularized tissue constructs 1 Dr. Raminder Singh
19:37 Oral Poster Diclofenac and moxifloxacin simultaneous delivery from intraocular lenses for endophthalmitis prophylaxis 1 Prof. Ana Serro
19:40 Oral Poster Optimization of a calcium phosphate based bone cement formulation 1 Prof. Dr. Rogério Cola?o
19:43 Oral Poster Nanostructured bone apatite-like thin films for bone prostheses 1 Dr. Gabriela Graziani
19:46 Oral Poster Low-dose BMP-2 enhances the bone-forming effects of an injectable, PLGA fiber-reinforced, brushite-forming cement in sheep lumbar osteopenia defects 1 Francesca Gunnella
19:49 Oral Poster The PLGA fiber component of brushite-forming calcium phosphate cement induces the osteogenic differentiation of human adipose tissue-derived stem cells 1 Francesca Gunnella
19:52 Oral Poster Surface functionalization of nanofibers by plasma processing in Ar/CO2/C2H4 discharge 0 Ph.D. Elizaveta Permyakova
19:55 Oral Poster Three-dimensional nanoscale X-ray computed tomography for the visualisation of cells grown on fibrous scaffolds 1 Dr. Juliana Martins de Souza e Silva

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Objectives: A brushite-forming calcium phosphate cement (CPC) stabilized by poly (l-lactide-co-glycolide) acid (PLGA) fibers (≤ 10% w/w) showed high biocompatibility and its fiber component enhanced bone formation in sheep lumbar vertebroplasty. However, CPC effects on the osteogenic differentiation of resident mesenchymal stem cells remained unexplored. Thus, the present study analyzed the influence of a CPC scaffold with a low PLGA proportion (as a mimicry of natural bone) on pluripotency, and osteogenic, chondrogenic, and adipogenic differentiation of human adipose tissue-derived mesenchymal stem cells (hASCs).

Methods: hASCs were cultivated on CPC discs with/without PLGA fibers (5 and 10%) in the absence of osteogenic medium for 3, 7, and 14 days. Gene expression of pluripotency (Nanog, Tert, Rex), osteogenic (Runx2, osterix, alkaline phosphatase, collagen I, osteonectin, osteopontin, osteocalcin), chondrogenic (collagen II, Sox9, aggrecan), and adipogenic markers (PPARG, Leptin, and FABP4) was analyzed by RT-PCR, alkaline phosphatase (ALP) activity by enzme assay, and cell number and viability by fluorescein diacetate/propidium iodide staining.

Results: Compared to pure CPC, hASCs cultured on fiber-reinforced CPC showed transiently induced gene expression of the osteogenic transcription factors Runx2 and osterix (day 3), and long-lastingly augmented expression of the bone markers alkaline phosphatase (including its activity), collagen I, and osteonectin (until day 14). In contrast, augmented expression of all chondrogenic, adipogenic and pluripotency markers was limited to day 3, followed by a significant downregulation on days 7 and 14. Cultivation of hASCs on fiber-reinforced CPC reduced the cell number, but not the proportion of viable cells (viability > 95%).

Conclusions: The PLGA component of fiber-reinforced, brushite-forming CPC supports long-lasting osteogenic differentiation of hASCs, whereas chondrogenesis, adipogenesis, and pluripotency are only transiently augmented. In parallel to the augmentation of bone formation by fiber addition in vivo, PLGA fibers may represent an interesting clinical target for future improvement of CPC-based bone regeneration.

Francesca Gunnella
University Hospital Jena
Additional Authors:
  • Dr. Elke Kunisch
    Universitätsklinikum Jena
  • Stefanie Wagner
    University Hospital Jena
  • Dr. Franziska Dees
    Alere Technologies GmbH
  • Dr. Stefan Maenz
    Aesculap AG
  • Dr.-Ing. Jörg Bossert
    Friedrich-Schiller-Universität Jena
  • Prof. Dr. Klaus D. Jandt
    Friedrich-Schiller-Universität Jena
  • Prof. Dr. Raimund W. Kinne
    Universitätsklinikum Jena