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Oral Poster

Three-dimensional nanoscale X-ray computed tomography for the visualisation of cells grown on fibrous scaffolds

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18:40 Oral Poster Effect of surface processing on roughness, wettability and cytotoxicity of carbon fiber-reinforced polyetheretherketone (PEEK) composite fabricated by fused deposition modeling 1 Dr. Xingting Han
18:43 Oral Poster Characterisation of bactericidal titanium surfaces using electron microscopy 1 Ph.D. Joshua Jenkins
18:49 Oral Poster Parvovirus B19 virus-like particles as platform for the development of biomaterials. 1 Prof. Dr. Ismael Bustos-Jaimes
18:52 Oral Poster The Influence of Surface Nanopatterns with varying Periodicities on Protein adsorption 1 Yu Yang
18:55 Oral Poster Opaque, Translucent and Transparent Flower Petals 1 Takumi Arakawa
18:58 Oral Poster Impact of Surface Morphology and Net Charge of Spider Silk based Materials on Interactions with Human Blood Components 1 Sarah Lentz
19:01 Oral Poster Modulation of Bacteria on Titanium Surfaces via Nanotopography and Antimicrobial Peptides 1 Marcus Eales
19:04 Oral Poster Degradable hybrid polymers for tissue engineering 1 Johannes Schwaiger
19:07 Oral Poster Antibacterial functionalization of plasma-chemical oxidized titanium surfaces 1 Dr. Stefan Kranz
19:10 Oral Poster Thermal crosslinking of hydrophilic polymers using polyperoxides 1 Ph.D. Solomiia Borova
19:13 Oral Poster Poly(peptoid)s: Peptidomimetics as potential Biomaterials 1 Christine Schlutt
19:16 Oral Poster A Nanoscaled Hybrid Coating for Neuronal Electrodes: Nanoporous Silica NPs Embodied into Nanoporous Platinum. 1 Tim-Joshua Pinkvos
19:19 Oral Poster Drug Delivery from Nanoporous Titania Coatings for Dental Application 1 Saskia Zailskas
19:22 Oral Poster Waste to resource: Biopolymer from industrial wastewater 1 Prof. Regina Nogueira
19:25 Oral Poster Divide and Comprehend: Separating the Effects of Hydrophobicity / Hydrophilicity and Crystallinity on the Thermal Properties of Polyester Based Drug Delivery Systems 1 Karl Scheuer
19:28 Oral Poster Elastic Polymer-Coated Nano- and Macroporous Bioactive Glass-Composites for Bone Regeneration 1 Marvin Lietzow
19:34 Oral Poster Alginate/silk fibroin-based hydrogels for vascularized tissue constructs 1 Dr. Raminder Singh
19:37 Oral Poster Diclofenac and moxifloxacin simultaneous delivery from intraocular lenses for endophthalmitis prophylaxis 1 Prof. Ana Serro
19:40 Oral Poster Optimization of a calcium phosphate based bone cement formulation 1 Prof. Dr. Rogério Cola?o
19:43 Oral Poster Nanostructured bone apatite-like thin films for bone prostheses 1 Dr. Gabriela Graziani
19:46 Oral Poster Low-dose BMP-2 enhances the bone-forming effects of an injectable, PLGA fiber-reinforced, brushite-forming cement in sheep lumbar osteopenia defects 1 Francesca Gunnella
19:49 Oral Poster The PLGA fiber component of brushite-forming calcium phosphate cement induces the osteogenic differentiation of human adipose tissue-derived stem cells 1 Francesca Gunnella
19:52 Oral Poster Surface functionalization of nanofibers by plasma processing in Ar/CO2/C2H4 discharge 0 Ph.D. Elizaveta Permyakova
19:55 Oral Poster Three-dimensional nanoscale X-ray computed tomography for the visualisation of cells grown on fibrous scaffolds 1 Dr. Juliana Martins de Souza e Silva

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Fibrous non-woven biomaterials are used in tissue engineering as biomimetic three-dimensional (3D) scaffolds to assist the growth of new tissues.[1] These fibers’ properties, such as their morphology, surface patterning, their pores’ size, and interconnectivity, have a great impact on the cellular growth[2] and, thus, are critical parameters for their successful application as scaffolds for tissue regeneration. Here we studied the influence of the properties of the fibers on the cellular growth behavior. We used electrospinning to prepare the fiber matrices that we cross-linked with formaldehyde. Afterwards, we used the matrices as scaffolds to assist the growth of fibroblasts. We characterized the 3D structure of the fibers matrices with nanoscale X-ray computed tomography (nano-CT) imaging and we found a decline of porosity of more than 20 %, and of the surface area of almost 40 % after 2 h of cross-linking time. We seeded the cells on the different matrices in parallel experiments, and we imaged the samples with nano-CT after staining them. Nano-CT imaging enabled the visualisation of the cells, and not only revealed their morphology, but also show that their porosity plays an important role in the pattern of the cells growth.

[1] D. Aibibu, M. Hild, et al., J. Mater. Sci.: Mater. Med. (2016) 27: 63. 
https://doi.org/10.1007/s10856-015-5656-3

[2] J. Rnjak-Kovacina, A. S. Weiss., Tissue Eng Part B: Rev. (2011) 17: 5.
http://doi.org/10.1089/ten.teb.2011.0235

Speaker:
Dr. Juliana Martins de Souza e Silva
Martin Luther University Halle-Wittenberg
Additional Authors:
  • Dr. Cristine Santos de Oliveira
    Martin-Luther-Universität Halle-Wittenberg
  • Adriana Trompetero González
    Martin-Luther-Universität Halle-Wittenberg
  • Tobias Kürbitz
    Anhalt University of Applied Science
  • Tobias Hedke
    Fraunhofer Institute for Microstructure of Materials and Systems IMWS
  • Dr. Christian E H Schmelzer
    Fraunhofer Institute for Microstructure of Materials and Systems IMWS
  • Prof. Dr. Ralf B. Wehrspohn
    Fraunhofer Institute for Microstructure of Materials and Systems IMWS