Creating standardized wells on solid biomaterials for biocompatibility testingWednesday (08.05.2019) 12:00 - 12:20 Part of:
For biocompatibility tests scientists rely on standardized, biocompatible plastic cell culture plates with known geometry and size of culture area. However, testing of solid biomaterial and implant material in this standardized plates is often challenging. First the biomaterial has to fit in the standardized well size. One major disadvantage of placing the biomaterial into a well is that plastic culture area surrounding the biomaterial is left and needs to be taken into account when evaluating the collected data. Additionally accurate prediction of cellular behavior around the edges and the bottom side of the biomaterial is complicated. Known solutions for creating wells on materials are often limited to smooth surfaces, like plastic or glass. In this work, we introduce our patented solution for creating standardized wells, which are fully comparable to cell culture well geometry, on a wide range of solid biomaterials. The system is adaptable to different material sizes and roughnesses as well as standard plastic and glass slides. Applications like direct cell contact assays, cell secretion assays or protein adsorption assays are easy to handle in our device thereby allowing replicable and multiple assaying on the same biomaterial. Here we describe the use of our patented device for the quantitative viability testing of cells in direct contact and limited to the biomaterial surface.