Staphylococcus aureus is one the most clinical relevant pathogen in implant-associated infections. The aim of the present study was to develop antibacterial self-active implant surfaces with high biocompatibility.
Plasma-chemical oxidized titanium (TiOB) was functionalized with gentamicin-tannic acid (TiOB gta), silver nanoparticles (TiOB SiOx Ag) and ionic zinc (TiOB Zn) by different techniques. The antibacterial effect on Staph. aureus was determined by agar diffusion and proliferation test. Cytocompatibility was analyzed by direct contact test on MC3T3-E1 cells (2d, 4d).
All surfaces showed significant antibacterial activity. In the proliferation assay extended lag phases were observed (TiOB gta 5 h, TiOB SiOx Ag 8 h, TiOB Zn 10 h). TiOB gta and TiOB Zn samples showed significant bacterial suppression of Staph. aureus up to 48 h. In contrast, TiOB SiOx Ag was bactericidal for only 4 h. After cultivation for 4d vital MC3T3-E1 cells were observed on all surfaces.
The present study shows that functionalization of TiOB with gentamicin and ionic Zn is efficient in suppressing Staph. aureus. Further investigations are necessary to determine the efficiency of these surfaces also in-vivo.