Blood plasma like material for multiphoton polymerizationThursday (09.05.2019) 12:10 - 12:30 Part of:
Multiphoton polymerization allows the production of complex, freestanding 3D structures with resolutions below the diffraction limit. No other technology today matches these characteristics. The reason for the high resolution is the underlying process of multiphoton absorption, which can only take place in the focus of the used femtosecond-pulse-laser. Multiphoton polymerization therefore provides unique opportunities especially in the field of tissue engineering. In our work we produced a solution with an ionic strength similar to that of human blood plasma but without any coagulation factors. It was shown, that exposure to the laser light under the given parameters, leads to a crosslinking of the mixture only in the periphery of the written structure. In further experiments the exact voxel dimensions were determined and different process parameters were investigated with the goal to produce a completely raised structure in the desired shape. At the same time the reaction mechanism in the solution, which is triggered by laser exposure, was examined in greater detail. Experiments with different cell lines can show, if the fabricated constructs are suitable for cell adhesion. The so called synthetic blood plasma is thus a new material which can have different applications in tissue engineering.