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Oral Poster

Alginate/silk fibroin-based hydrogels for vascularized tissue constructs

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18:40 Oral Poster Effect of surface processing on roughness, wettability and cytotoxicity of carbon fiber-reinforced polyetheretherketone (PEEK) composite fabricated by fused deposition modeling 1 Dr. Xingting Han
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18:52 Oral Poster The Influence of Surface Nanopatterns with varying Periodicities on Protein adsorption 1 Yu Yang
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19:01 Oral Poster Modulation of Bacteria on Titanium Surfaces via Nanotopography and Antimicrobial Peptides 1 Marcus Eales
19:04 Oral Poster Degradable hybrid polymers for tissue engineering 1 Johannes Schwaiger
19:07 Oral Poster Antibacterial functionalization of plasma-chemical oxidized titanium surfaces 1 Dr. Stefan Kranz
19:10 Oral Poster Thermal crosslinking of hydrophilic polymers using polyperoxides 1 Ph.D. Solomiia Borova
19:13 Oral Poster Poly(peptoid)s: Peptidomimetics as potential Biomaterials 1 Christine Schlutt
19:16 Oral Poster A Nanoscaled Hybrid Coating for Neuronal Electrodes: Nanoporous Silica NPs Embodied into Nanoporous Platinum. 1 Tim-Joshua Pinkvos
19:19 Oral Poster Drug Delivery from Nanoporous Titania Coatings for Dental Application 1 Saskia Zailskas
19:22 Oral Poster Waste to resource: Biopolymer from industrial wastewater 1 Prof. Regina Nogueira
19:25 Oral Poster Divide and Comprehend: Separating the Effects of Hydrophobicity / Hydrophilicity and Crystallinity on the Thermal Properties of Polyester Based Drug Delivery Systems 1 Karl Scheuer
19:28 Oral Poster Elastic Polymer-Coated Nano- and Macroporous Bioactive Glass-Composites for Bone Regeneration 1 Marvin Lietzow
19:34 Oral Poster Alginate/silk fibroin-based hydrogels for vascularized tissue constructs 1 Dr. Raminder Singh
19:37 Oral Poster Diclofenac and moxifloxacin simultaneous delivery from intraocular lenses for endophthalmitis prophylaxis 1 Prof. Ana Serro
19:40 Oral Poster Optimization of a calcium phosphate based bone cement formulation 1 Prof. Dr. Rogério Cola?o
19:43 Oral Poster Nanostructured bone apatite-like thin films for bone prostheses 1 Dr. Gabriela Graziani
19:46 Oral Poster Low-dose BMP-2 enhances the bone-forming effects of an injectable, PLGA fiber-reinforced, brushite-forming cement in sheep lumbar osteopenia defects 1 Francesca Gunnella
19:49 Oral Poster The PLGA fiber component of brushite-forming calcium phosphate cement induces the osteogenic differentiation of human adipose tissue-derived stem cells 1 Francesca Gunnella
19:52 Oral Poster Surface functionalization of nanofibers by plasma processing in Ar/CO2/C2H4 discharge 0 Ph.D. Elizaveta Permyakova
19:55 Oral Poster Three-dimensional nanoscale X-ray computed tomography for the visualisation of cells grown on fibrous scaffolds 1 Dr. Juliana Martins de Souza e Silva

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Introduction: Development of tissue grafts and in vitro tissue models is hindered by the lack of vascular networks formation in the engineered constructs. In this study, we used primary human cells to investigate whether hydrogels based on alginate (Alg) blended with silk fibroin (SF) support the spontaneous formation of the tubular networks.

Methods: Alg/SF hydrogels containing normal human dermal fibroblasts were placed in the 24-well plates and cultivated for 14 days. Afterwards, human umbilical vein endothelial cells (HUVEC) loaded with magnetic particles (SPIONs, 3 µg/cm2) were seeded on the hydrogels, in the presence of magnetic field during the first 24 h. The growth of cells was monitored for additional 3 weeks. Metabolic activity of the cells was measured using WST-8 assay. The distribution of HUVECs and fibroblasts was investigated using CD31 or N-Cadherin staining. Two-photon microscopy was used for 3D images and to detect collagen formation (Collagen Iα1 antibody).

Results: Compared with fibroblast only, hydrogels containing both HUVECs and fibroblasts had higher metabolic activity from day 7 of co-culture onwards. HUVECs did not remain on the surface of hydrogels and gradually migrated inside the films. The formation of neo-vessel like structures (N-Cadherin + CD31) was detectable from day 20 of co-culture. Collagen, which is present in all connective tissues and is an indicator of the extracellular matrix (ECM) formation, was detected in the hydrogels at day 20 of fibroblast and HUVEC co-culture.

Conclusions: Alg/SF hydrogels support growth of different vascular cells, as well as the formation of ECM and vessel-like structures. Our initial investigations showed promising results with this highly-biocompatible scaffold, but further efforts are required to produce and validate the vascularized tissue constructs.

Dr. Raminder Singh
Universitätsklinikum Erlangen
Additional Authors:
  • Hatice Genc
    Universitätsklinikum Erlangen
  • Thomas Distler
    Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU)
  • Dr. Rainer Detsch
    Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU)
  • Dr. Barbara Dietel
    Universitätsklinikum Erlangen
  • Prof. Dr. Aldo Boccaccini
    Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU)
  • Prof. Dr. Christoph Alexiou
    Universitätsklinikum Erlangen
  • Dr. Iwona Cicha
    Universitätsklinikum Erlangen