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Poster

Nano-rough titanium surfaces created by wet alkaline etching and PVD and their influence on bacterial adhesion as well as protein adsorption

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Line-Up:
- Plenary Lecture Test Ploetz 1 Alexia Ploetz
- Poster Investigating Dynamic Biological Processes with High-Speed, High-Resolution Correlative AFM-Light Microscopy 1 Oilibhe Pabsch
- Poster Corrosion behaviour of potencially biodegradable ternary alloy Zn-Mg-Ca 1 Dipl.-Ing. Jan Pinc
- Poster Multi scale topography in titanium alloys by electron beam technique: influence on the tissue adhesion and bacterial contamination 1 Dr. Fernando Warchomicka
- Poster Cytotoxicity of gold nanorods and nanoribbons in context of biomedical applications 1 Marika Musielak
- Poster Preliminary studies of synthesis of gold nanoribbons using selected dicationic surfactants 1 Joanna Patalas
- Poster Diclofenac release from a silicon based hydrogel for contact lenses controlled by a layer-by-layer coating 1 Prof. Ana Serro
- Poster Synthesis, solubility and optical activity of chiral poly(2,4-disubstituted-2-oxazoline)s 1 Mengshi Yang
- Poster Influence of Cu2+-doping in β-TCP on the hydration kinetics of brushite cement investigated by Rietveld analysis and heat flow calorimetry 1 Karla Späth
- Poster Analytical investigation of the influence of micro- and nanoplastic particles in crop plants by electron microscopic imaging and spectroscopy 1 Dr. Jörg Wissler
- Poster Influence of fluoride corrosion attack on the metal-porcelain bond strength of TiCr20 compared to titanium 1 Christine Schille
- Poster Bacterial nanocellulose loaded with frankincense extract as natural wound dressing to treat local dermal inflammations 1 Berit Karl
- Poster Graded functionalization of polydopamine coated fiber mats as potential implant for tendon-bone-transition 1 Sarah Oehmichen
- Poster Enhancing the functionalities of bioactive glass-based scaffolds by novel polymeric coatings 1 Dr. Liliana Liverani
- Poster Durability analysis of the cryopreservation of DNA origami nanostructures 1 Yang Xin
- Poster Effects of staple age on DNA origami nanostructure assembly and stability 1 Charlotte Kielar
- Poster Dual-setting brushite-silica gel cements – Variations of the monomer precursors and tuning of the setting parameters 1 Ib Holzmeister
- Poster Novel QCM-D/microscopy set-up for real-time analysis of bacterial binding onto biomaterial-coated sensors 1 Dr. Zeqian Xu
- Poster Chairside plasma activation of encapsulated implants: how fast is it? 1 Miguel Angel Garcia Chame
- Poster Nanostructured high-pressure laminate surfaces to reduce transmission pathways of pathogens in public transportation 1 Christian Helbing
- Poster Biomineralization of Recombinant Spider Silk Films 1 Vanessa J. Neubauer
- Poster Modification of bacterial cellulose to enhance anti-inflammatory wound dressing properties 1 Uwe Beekmann
- Poster Absolute Characterization of Synthetic Macromolecules used in Biomaterials Science Applications 1 Mandy Grube
- Poster Biodegradable thermoplastic materials for bimodal imaging containing the mineral present in the human body 1 Zaneta Górecka
- Poster Nano-rough titanium surfaces created by wet alkaline etching and PVD and their influence on bacterial adhesion as well as protein adsorption 1 Michaela Schürmann
- Poster Effective strategies for the controlling and dealing with transmission pathway of pathogens in air traffi 1 Christian Helbing
- Poster Cytotoxicity evaluation of biodegradable Zn-4Cu alloy using L929, Saos-2 and TAg cell lines 1 Ping Li
- Poster Influence of pulsed bias voltage on the tribological and morphological properties of a-C coatings deposited by an anodic arc method 1 Dipl.-Ing. Ingo Erdmann

Session P: Poster Session
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Biomaterials-associated infections are most frequently caused by the colonization of microorganisms on the materials’ surface. In the form of biofilms, bacterial cells are highly resistant against biocides and mechanical stress. Previously, it was shown that titanium surfaces with a nano-roughness in the range of 2 – 6 nm have the potential to reduce microbial adhesion. The nano-roughness of our previous approach is limited due to the instrumental restriction of physical vapor deposition (PVD). We aim to extend this research by examining the previously uninvestigated roughness range of Ra= 2 – 16 nm. Therefore, we modified the previous approach to a combination of PVD and wet etching. Additionally, we wish to understand how the nano-roughness influences microbial adhesion as well as protein adsorption.

Titanium thin films, as prepared by PVD, were consequently treated in NaOH solution with tunable concentration, in order to achieve surfaces with an extended nano-roughness range from 6 to 24 nm. Through a second titanium layer prepared by the PVD, uniform chemical properties of the different rough surfaces have been ensured. Atomic force microscopy (AFM), scanning electron microscopy (SEM) as well as x-ray photoelectron spectroscopy (XPS) were used to characterize the surfaces in terms of roughness, morphology and chemical properties. Also bacterial adhesion tests with Escherichia coli for different adhesion times and protein (albumin and fibrinogen) adsorption tests with different concentrations were done. Fluorescence microscopy and SEM were used to characterize the bacterial adhesion tests and AFM and µBCA were used to measure the protein adsorption.

We present a summary of our results of nano-rough titanium thin films produced with this method. It is possible to create roughness through etching in the predefined range. The SEM images show similar morphologies to surfaces prepared only by the PVD. Similarly, the chemical properties are the same after the second round of PVD. Also we present our first results of the microbial adhesion and protein adsorption tests.

These results show that this method is suitable to produce nano-rough titanium surfaces in the required range. This is the foundation for investigating the influence of nano-rough surfaces on microbial adhesion.

We thank the DFG for funding of our project: “Antimicrobial Effect of Nano-rough Titanium surfaces: Reduction of Microbial Adhesion and Mechanisms of Reduction” AOBJ: 622946.

Speaker:
Michaela Schürmann
Friedrich Schiller University Jena
Additional Authors:
  • Nathalie Stefani
    Leibniz Institute for Natural Product Research and Infection Biology
  • Dr. Volker Schroekh
    Leibniz Institute for Natural Product Research and Infection Biology
  • Prof. Dr. Axel A. Brakhage
    Leibniz Institute for Natural Product Research and Infection Biology
  • Dr. Jörg Bossert
    Friedrich Schiller University Jena
  • Prof. Dr. Klaus D. Jandt
    Friedrich Schiller University Jena