Plant-Derived Scaffolds for Tissue Engineering ApplicationsWednesday (08.05.2019) 14:50 - 15:10 Part of:
The main purpose of tissue engineering is to regenerate damaged tissues by using three-dimensional (3D) scaffold which mimics the in vivo cellular milieu. The major problem with this approach is that the need of human donors and animal products exceeds the availability1. Recently, cellulose-based scaffolds that are easily attainable in nature and quite convenient for the mammalian cell culture have been studied2,3. Plant-derived scaffolds have great potential for various tissue engineering applications including neural tissue engineering. In this study, leek was chosen as a model plant tissue due to its structural morphology (interconnected and elongated channel like structures) for neural tissue engineering applications. Firstly, leek tissues were treated with low concentration aqueous detergent to disrupt the nucleus and wash the cell content. After removal of excess detergent thoroughly, DNA and protein quantification of the decellularized samples were measured in order to evaluate the degree of decellularization along with fluorescence staining of key cell organelles. Tensile strength and elastic modulus were measured to assess the mechanical properties of plant tissues that were decellularized. SH-SY5Y human neuroblastoma cell line was used as mammalian cell source and seeded onto the decellularized leek tissues. Chemical structures were analyzed by Fourier Transform Infrared Spectroscopy (FT-IR). Morphological characterization of remained scaffolds and cell adhesion were surveyed by Scanning Electron Microscope (SEM) to image the surface topography. MTT assay was done in order to observe cell viability and proliferation; also Acridine Orange/Propidium Iodide (AO/PI) staining was performed to show live and dead cells. Prepared decellularized plant tissues are expected to be candidate scaffolds for neural tissue engineering applications in-vitro and in-vivo conditions as future studies.