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Poster

Novel QCM-D/microscopy set-up for real-time analysis of bacterial binding onto biomaterial-coated sensors

Part of:
Line-Up:
- Plenary Lecture Test Ploetz 1 Alexia Ploetz
- Poster Investigating Dynamic Biological Processes with High-Speed, High-Resolution Correlative AFM-Light Microscopy 1 Oilibhe Pabsch
- Poster Corrosion behaviour of potencially biodegradable ternary alloy Zn-Mg-Ca 1 Dipl.-Ing. Jan Pinc
- Poster Multi scale topography in titanium alloys by electron beam technique: influence on the tissue adhesion and bacterial contamination 1 Dr. Fernando Warchomicka
- Poster Cytotoxicity of gold nanorods and nanoribbons in context of biomedical applications 1 Marika Musielak
- Poster Preliminary studies of synthesis of gold nanoribbons using selected dicationic surfactants 1 Joanna Patalas
- Poster Diclofenac release from a silicon based hydrogel for contact lenses controlled by a layer-by-layer coating 1 Prof. Ana Serro
- Poster Synthesis, solubility and optical activity of chiral poly(2,4-disubstituted-2-oxazoline)s 1 Mengshi Yang
- Poster Influence of Cu2+-doping in β-TCP on the hydration kinetics of brushite cement investigated by Rietveld analysis and heat flow calorimetry 1 Karla Späth
- Poster Analytical investigation of the influence of micro- and nanoplastic particles in crop plants by electron microscopic imaging and spectroscopy 1 Dr. Jörg Wissler
- Poster Influence of fluoride corrosion attack on the metal-porcelain bond strength of TiCr20 compared to titanium 1 Christine Schille
- Poster Bacterial nanocellulose loaded with frankincense extract as natural wound dressing to treat local dermal inflammations 1 Berit Karl
- Poster Graded functionalization of polydopamine coated fiber mats as potential implant for tendon-bone-transition 1 Sarah Oehmichen
- Poster Enhancing the functionalities of bioactive glass-based scaffolds by novel polymeric coatings 1 Dr. Liliana Liverani
- Poster Durability analysis of the cryopreservation of DNA origami nanostructures 1 Yang Xin
- Poster Effects of staple age on DNA origami nanostructure assembly and stability 1 Charlotte Kielar
- Poster Dual-setting brushite-silica gel cements – Variations of the monomer precursors and tuning of the setting parameters 1 Ib Holzmeister
- Poster Novel QCM-D/microscopy set-up for real-time analysis of bacterial binding onto biomaterial-coated sensors 1 Dr. Zeqian Xu
- Poster Chairside plasma activation of encapsulated implants: how fast is it? 1 Miguel Angel Garcia Chame
- Poster Nanostructured high-pressure laminate surfaces to reduce transmission pathways of pathogens in public transportation 1 Christian Helbing
- Poster Biomineralization of Recombinant Spider Silk Films 1 Vanessa J. Neubauer
- Poster Modification of bacterial cellulose to enhance anti-inflammatory wound dressing properties 1 Uwe Beekmann
- Poster Absolute Characterization of Synthetic Macromolecules used in Biomaterials Science Applications 1 Mandy Grube
- Poster Biodegradable thermoplastic materials for bimodal imaging containing the mineral present in the human body 1 Zaneta Górecka
- Poster Nano-rough titanium surfaces created by wet alkaline etching and PVD and their influence on bacterial adhesion as well as protein adsorption 1 Michaela Schürmann
- Poster Effective strategies for the controlling and dealing with transmission pathway of pathogens in air traffi 1 Christian Helbing
- Poster Cytotoxicity evaluation of biodegradable Zn-4Cu alloy using L929, Saos-2 and TAg cell lines 1 Ping Li
- Poster Influence of pulsed bias voltage on the tribological and morphological properties of a-C coatings deposited by an anodic arc method 1 Dipl.-Ing. Ingo Erdmann

Session P: Poster Session
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Background and aims:

Mass-sensitive quartz crystal microbalance with dissipation monitoring (QCM-D) is widely used in sensing bacteria binding. Studies indicate, that due to dissipative bacterial binding, mass loading cannot be directly calculated by the classical Sauerbrey equation. This study aimed at correlating the number of surface-bound bacteria with simultaneously detected frequency and dissipation signals to improve QCM-D data interpretation.


Materials and methods:

A QCM-D flow chamber device (qCell T μOpto; 3T analytik) with an optic window and a titanium (Ti) coated quartz sensor was combined with a fluorescent microscope. The early oral colonizer Streptococcus gordonii was cultured in Schaedler Medium, harvested by centrifugation, S. gordonii were stained with SYTO®9 and diluted with PBS to OD620 =0.1. After reaching stable signal baselines with PBS, the stained bacteria were pumped with 60 μl/min through the system for 3 hours, followed by 30 min PBS rinsing. Fluorescent microscopic images were made every 5 min. The bacterial number was counted with Image J and correlated with the frequency and dissipation signal through q viewer 18.0.


Results:

The 3-hour S. gordonii runs ended with a small increase of both frequency (ΔF=+37.7Hz) and dissipation (ΔD=+72.4Hz). The number of surface bound S. gordonii were found to be positively correlated with both frequency and dissipation with a better correlation for the dissipation.


Conclusions:

A mass loss was detected by QCM-D during S. gordonii binding even though microscopically an increase of the number of attached bacteria was observed. This frequency increase can be interpreted here by the coupled oscillator model and indicated a soft, punctual binding of the bacteria. The QCM-D/microscopy set-up presented here can be used also for online testing of antimicrobial agents and surfaces.

 

Speaker:
Dr. Zeqian Xu
University Hospital Tübingen
Additional Authors:
  • Dr. Thomas Reiner
    3T analytik
  • Dr. Lutz Scheideler
    University Hospital Tübingen
  • Dr. Stefanie Krajewski
    University Hospital Tübingen
  • Prof. Dr. Juergen Geis-Gerstorfer
    University Hospital Tübingen
  • Dr. Frank Gehring
    3T analytik
  • Prof. Dr. Frank Rupp
    University Hospital Tübingen