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Oral Poster

Impact of Surface Morphology and Net Charge of Spider Silk based Materials on Interactions with Human Blood Components

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Line-Up:
18:40 Oral Poster Effect of surface processing on roughness, wettability and cytotoxicity of carbon fiber-reinforced polyetheretherketone (PEEK) composite fabricated by fused deposition modeling 1 Dr. Xingting Han
18:43 Oral Poster Characterisation of bactericidal titanium surfaces using electron microscopy 1 Ph.D. Joshua Jenkins
18:49 Oral Poster Parvovirus B19 virus-like particles as platform for the development of biomaterials. 1 Prof. Dr. Ismael Bustos-Jaimes
18:52 Oral Poster The Influence of Surface Nanopatterns with varying Periodicities on Protein adsorption 1 Yu Yang
18:55 Oral Poster Opaque, Translucent and Transparent Flower Petals 1 Takumi Arakawa
18:58 Oral Poster Impact of Surface Morphology and Net Charge of Spider Silk based Materials on Interactions with Human Blood Components 1 Sarah Lentz
19:01 Oral Poster Modulation of Bacteria on Titanium Surfaces via Nanotopography and Antimicrobial Peptides 1 Marcus Eales
19:04 Oral Poster Degradable hybrid polymers for tissue engineering 1 Johannes Schwaiger
19:07 Oral Poster Antibacterial functionalization of plasma-chemical oxidized titanium surfaces 1 Dr. Stefan Kranz
19:10 Oral Poster Thermal crosslinking of hydrophilic polymers using polyperoxides 1 Ph.D. Solomiia Borova
19:13 Oral Poster Poly(peptoid)s: Peptidomimetics as potential Biomaterials 1 Christine Schlutt
19:16 Oral Poster A Nanoscaled Hybrid Coating for Neuronal Electrodes: Nanoporous Silica NPs Embodied into Nanoporous Platinum. 1 Tim-Joshua Pinkvos
19:19 Oral Poster Drug Delivery from Nanoporous Titania Coatings for Dental Application 1 Saskia Zailskas
19:22 Oral Poster Waste to resource: Biopolymer from industrial wastewater 1 Prof. Regina Nogueira
19:25 Oral Poster Divide and Comprehend: Separating the Effects of Hydrophobicity / Hydrophilicity and Crystallinity on the Thermal Properties of Polyester Based Drug Delivery Systems 1 Karl Scheuer
19:28 Oral Poster Elastic Polymer-Coated Nano- and Macroporous Bioactive Glass-Composites for Bone Regeneration 1 Marvin Lietzow
19:34 Oral Poster Alginate/silk fibroin-based hydrogels for vascularized tissue constructs 1 Dr. Raminder Singh
19:37 Oral Poster Diclofenac and moxifloxacin simultaneous delivery from intraocular lenses for endophthalmitis prophylaxis 1 Prof. Ana Serro
19:40 Oral Poster Optimization of a calcium phosphate based bone cement formulation 1 Prof. Dr. Rogério Cola?o
19:43 Oral Poster Nanostructured bone apatite-like thin films for bone prostheses 1 Dr. Gabriela Graziani
19:46 Oral Poster Low-dose BMP-2 enhances the bone-forming effects of an injectable, PLGA fiber-reinforced, brushite-forming cement in sheep lumbar osteopenia defects 1 Francesca Gunnella
19:49 Oral Poster The PLGA fiber component of brushite-forming calcium phosphate cement induces the osteogenic differentiation of human adipose tissue-derived stem cells 1 Francesca Gunnella
19:52 Oral Poster Surface functionalization of nanofibers by plasma processing in Ar/CO2/C2H4 discharge 0 Ph.D. Elizaveta Permyakova
19:55 Oral Poster Three-dimensional nanoscale X-ray computed tomography for the visualisation of cells grown on fibrous scaffolds 1 Dr. Juliana Martins de Souza e Silva

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Materials made of recombinant spider silk are promising candidates for a broad range of applications in the field of biomedicine 1, because of their mechanical and chemical properties.2 Further, spider silk shows non-inflammatory and hypoallergenic properties.2 For biomaterials it is of utmost importance to evaluate their interactions with blood proteins.3 Therefore, the main objective of this research is to investigate the interactions of human plasma and human blood with planar spider silk films and spherical spider silk particles. These two morphologies are made of recombinantly produced spider silk proteins, which can be easily modified concerning their net charge and morphology, and they are examined in terms of potential blood clotting alongside with fibrin formation.4, 5 Further, protein adhesion studies and in-depth proteomic analysis is performed and highlighting the impact of surface morphology and charge on interactions with human blood components.


References

1. Borkner, C. B.; Elsner, M. B.; Scheibel, T., Coatings and Films Made of Silk Proteins. ACS Applied Materials & Interfaces 2014, 6 (18), 15611-15625.

2. Doblhofer, E.; Heidebrecht, A.; Scheibel, T., To spin or not to spin: spider silk fibers and more. Appl. Microbiol. Biotechnol. 2015, 99 (22), 9361-9380.

3. Mertgen, A.-S.; Yazgan, G.; Guex, A. G.; Fortunato, G.; Müller, E.; Huber, L.; Schneider, R.; Brunelli, M.; Rossi, R. M.; Maniura-Weber, K.; Rottmar, M., Controlling the surface structure of electrospun fibers: Effect on endothelial cells and blood coagulation. 2018, 13 (5), 051001.

4. Huemmerich, D.; Helsen, C. W.; Quedzuweit, S.; Oschmann, J.; Rudolph, R.; Scheibel, T., Primary Structure Elements of Spider Dragline Silks and Their Contribution to Protein Solubility. Biochemistry 2004, 43 (42), 13604-13612.

5. Doblhofer, E.; Scheibel, T., Engineering of Recombinant Spider Silk Proteins Allows Defined Uptake and Release of Substances. Journal of Pharmaceutical Sciences 2015, 104 (3), 988-994.

 

Speaker:
Sarah Lentz
University of Bayreuth
Additional Authors:
  • Dr. Alessia C. G. Weiss
    The University of Melbourne
  • Heike Herold
    University of Bayreuth
  • Dr. Matt Faria
    The University of Melbourne
  • Dr. Quinn Besford
    The University of Melbourne
  • Chrstian Borkner
    University of Bayreuth
  • Ching-Seng Ang
    The University of Melbourne
  • Dr. Edmund J. Crampin
    The University of Melbourne
  • Dr. Eike Müller
    EMPA Swiss Federal Laboratories for Materials Science and Technology
  • Dr. Markus Rottmar
    EMPA Swiss Federal Laboratories for Materials Science and Technology
  • Prof. Dr. Frank Caruso
    The University of Melbourne
  • Prof. Dr. Thomas Scheibel
    University of Bayreuth