Materials made of recombinant spider silk are promising candidates for a broad range of applications in the field of biomedicine 1, because of their mechanical and chemical properties.2 Further, spider silk shows non-inflammatory and hypoallergenic properties.2 For biomaterials it is of utmost importance to evaluate their interactions with blood proteins.3 Therefore, the main objective of this research is to investigate the interactions of human plasma and human blood with planar spider silk films and spherical spider silk particles. These two morphologies are made of recombinantly produced spider silk proteins, which can be easily modified concerning their net charge and morphology, and they are examined in terms of potential blood clotting alongside with fibrin formation.4, 5 Further, protein adhesion studies and in-depth proteomic analysis is performed and highlighting the impact of surface morphology and charge on interactions with human blood components.
1. Borkner, C. B.; Elsner, M. B.; Scheibel, T., Coatings and Films Made of Silk Proteins. ACS Applied Materials & Interfaces 2014, 6 (18), 15611-15625.
2. Doblhofer, E.; Heidebrecht, A.; Scheibel, T., To spin or not to spin: spider silk fibers and more. Appl. Microbiol. Biotechnol. 2015, 99 (22), 9361-9380.
3. Mertgen, A.-S.; Yazgan, G.; Guex, A. G.; Fortunato, G.; Müller, E.; Huber, L.; Schneider, R.; Brunelli, M.; Rossi, R. M.; Maniura-Weber, K.; Rottmar, M., Controlling the surface structure of electrospun fibers: Effect on endothelial cells and blood coagulation. 2018, 13 (5), 051001.
4. Huemmerich, D.; Helsen, C. W.; Quedzuweit, S.; Oschmann, J.; Rudolph, R.; Scheibel, T., Primary Structure Elements of Spider Dragline Silks and Their Contribution to Protein Solubility. Biochemistry 2004, 43 (42), 13604-13612.
5. Doblhofer, E.; Scheibel, T., Engineering of Recombinant Spider Silk Proteins Allows Defined Uptake and Release of Substances. Journal of Pharmaceutical Sciences 2015, 104 (3), 988-994.