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Cytotoxicity evaluation of biodegradable Zn-4Cu alloy using L929, Saos-2 and TAg cell lines

Part of:
- Plenary Lecture Test Ploetz 1 Alexia Ploetz
- Poster Investigating Dynamic Biological Processes with High-Speed, High-Resolution Correlative AFM-Light Microscopy 1 Oilibhe Pabsch
- Poster Corrosion behaviour of potencially biodegradable ternary alloy Zn-Mg-Ca 1 Dipl.-Ing. Jan Pinc
- Poster Multi scale topography in titanium alloys by electron beam technique: influence on the tissue adhesion and bacterial contamination 1 Dr. Fernando Warchomicka
- Poster Cytotoxicity of gold nanorods and nanoribbons in context of biomedical applications 1 Marika Musielak
- Poster Preliminary studies of synthesis of gold nanoribbons using selected dicationic surfactants 1 Joanna Patalas
- Poster Diclofenac release from a silicon based hydrogel for contact lenses controlled by a layer-by-layer coating 1 Prof. Ana Serro
- Poster Synthesis, solubility and optical activity of chiral poly(2,4-disubstituted-2-oxazoline)s 1 Mengshi Yang
- Poster Influence of Cu2+-doping in β-TCP on the hydration kinetics of brushite cement investigated by Rietveld analysis and heat flow calorimetry 1 Karla Späth
- Poster Analytical investigation of the influence of micro- and nanoplastic particles in crop plants by electron microscopic imaging and spectroscopy 1 Dr. Jörg Wissler
- Poster Influence of fluoride corrosion attack on the metal-porcelain bond strength of TiCr20 compared to titanium 1 Christine Schille
- Poster Bacterial nanocellulose loaded with frankincense extract as natural wound dressing to treat local dermal inflammations 1 Berit Karl
- Poster Graded functionalization of polydopamine coated fiber mats as potential implant for tendon-bone-transition 1 Sarah Oehmichen
- Poster Enhancing the functionalities of bioactive glass-based scaffolds by novel polymeric coatings 1 Dr. Liliana Liverani
- Poster Durability analysis of the cryopreservation of DNA origami nanostructures 1 Yang Xin
- Poster Effects of staple age on DNA origami nanostructure assembly and stability 1 Charlotte Kielar
- Poster Dual-setting brushite-silica gel cements – Variations of the monomer precursors and tuning of the setting parameters 1 Ib Holzmeister
- Poster Novel QCM-D/microscopy set-up for real-time analysis of bacterial binding onto biomaterial-coated sensors 1 Dr. Zeqian Xu
- Poster Chairside plasma activation of encapsulated implants: how fast is it? 1 Miguel Angel Garcia Chame
- Poster Nanostructured high-pressure laminate surfaces to reduce transmission pathways of pathogens in public transportation 1 Christian Helbing
- Poster Biomineralization of Recombinant Spider Silk Films 1 Vanessa J. Neubauer
- Poster Modification of bacterial cellulose to enhance anti-inflammatory wound dressing properties 1 Uwe Beekmann
- Poster Absolute Characterization of Synthetic Macromolecules used in Biomaterials Science Applications 1 Mandy Grube
- Poster Biodegradable thermoplastic materials for bimodal imaging containing the mineral present in the human body 1 Zaneta Górecka
- Poster Nano-rough titanium surfaces created by wet alkaline etching and PVD and their influence on bacterial adhesion as well as protein adsorption 1 Michaela Schürmann
- Poster Effective strategies for the controlling and dealing with transmission pathway of pathogens in air traffi 1 Christian Helbing
- Poster Cytotoxicity evaluation of biodegradable Zn-4Cu alloy using L929, Saos-2 and TAg cell lines 1 Ping Li
- Poster Influence of pulsed bias voltage on the tribological and morphological properties of a-C coatings deposited by an anodic arc method 1 Dipl.-Ing. Ingo Erdmann

Session P: Poster Session
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A biodegradable Zinc-copper alloy (Zn-4wt.%Cu) with enhanced mechanical properties has been developed for potential use as craniomaxillofacial osteosynthesis implant material. In this study, three cell lines, namely L929 mouse fibroblasts, the human osteosarcoma cell line Saos-2, and a cell line of human immortalized cranial periosteal cells (TAg), were used to investigate cytotoxic effects of the Zn-4Cu alloy towards the respective cells in vitro.

An extract test was performed to evaluate cytotoxicity for both as-cast and as-rolled Zn-4Cu alloy, Ti-6Al-4V was used as negative control. Samples were extracted in the respective cell culture media without serum with a surface/volume ratio of 1.25 cm2/mL for 24 h. Metallic ion concentration and pH value of the extracts were determined. Cell morphology and attachment were qualitatively evaluated by live/dead staining. Inhibition of metabolic activity and cell proliferation were quantitatively assessed by CCK-8 assay and BrdU assay, respectively.

L929, Saos-2 and TAg cells exposed to the extracts showed a spindle-shaped attachment pattern, and cell morphologies were similar to the respective counterparts on the titanium controls. For as-cast and as-rolled Zn-4Cu alloys, the mean relative metabolic activities of all cells were always exceeding 70% of the controls, without statistically significant differences between both groups (p > 0.05). For Saos-2 and TAg cells, the as-rolled Zn-4Cu alloy even induced a statistically significant increase in cell proliferation compared to the negative control (p < 0.05).

The as-rolled Zn-4Cu alloy exhibited no obvious toxic effect towards L929, TAg and Saos-2 cells, probably due to the determined low Zn ion concentration in the extracts, which is well under cellular tolerance limits. Moreover, cell proliferation rates of TAg and Saos-2 were shown to be activated by specific Zn ion concentrations in the as-rolled Zn-4Cu alloy extracts. Nevertheless, further studies should analyze the in vivo biocompatibility of as-rolled Zn-4Cu alloys.

Ping Li
University Hospital Tübingen
Additional Authors:
  • Wentai Zhang
    Southwest Jiaotong University
  • Ernst Schweizer
    University Hospital Tübingen
  • Jingtao Dai
    University Hospital Tübingen
  • Prof. Dr. Dorothea Alexander
    University Hospital Tübingen
  • Prof. Dr. Guojiang Wan
    Southwest Jiaotong University
  • Prof. Dr. Jürgen Geis-Gerstorfer
    University Hospital Tübingen
  • Dr. Lutz Scheideler
    University Hospital Tübingen