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Oral Poster

Characterisation of bactericidal titanium surfaces using electron microscopy

Part of:
Line-Up:
18:40 Oral Poster Effect of surface processing on roughness, wettability and cytotoxicity of carbon fiber-reinforced polyetheretherketone (PEEK) composite fabricated by fused deposition modeling 1 Dr. Xingting Han
18:43 Oral Poster Characterisation of bactericidal titanium surfaces using electron microscopy 1 Ph.D. Joshua Jenkins
18:49 Oral Poster Parvovirus B19 virus-like particles as platform for the development of biomaterials. 1 Prof. Dr. Ismael Bustos-Jaimes
18:52 Oral Poster The Influence of Surface Nanopatterns with varying Periodicities on Protein adsorption 1 Yu Yang
18:55 Oral Poster Opaque, Translucent and Transparent Flower Petals 1 Takumi Arakawa
18:58 Oral Poster Impact of Surface Morphology and Net Charge of Spider Silk based Materials on Interactions with Human Blood Components 1 Sarah Lentz
19:01 Oral Poster Modulation of Bacteria on Titanium Surfaces via Nanotopography and Antimicrobial Peptides 1 Marcus Eales
19:04 Oral Poster Degradable hybrid polymers for tissue engineering 1 Johannes Schwaiger
19:07 Oral Poster Antibacterial functionalization of plasma-chemical oxidized titanium surfaces 1 Dr. Stefan Kranz
19:10 Oral Poster Thermal crosslinking of hydrophilic polymers using polyperoxides 1 Ph.D. Solomiia Borova
19:13 Oral Poster Poly(peptoid)s: Peptidomimetics as potential Biomaterials 1 Christine Schlutt
19:16 Oral Poster A Nanoscaled Hybrid Coating for Neuronal Electrodes: Nanoporous Silica NPs Embodied into Nanoporous Platinum. 1 Tim-Joshua Pinkvos
19:19 Oral Poster Drug Delivery from Nanoporous Titania Coatings for Dental Application 1 Saskia Zailskas
19:22 Oral Poster Waste to resource: Biopolymer from industrial wastewater 1 Prof. Regina Nogueira
19:25 Oral Poster Divide and Comprehend: Separating the Effects of Hydrophobicity / Hydrophilicity and Crystallinity on the Thermal Properties of Polyester Based Drug Delivery Systems 1 Karl Scheuer
19:28 Oral Poster Elastic Polymer-Coated Nano- and Macroporous Bioactive Glass-Composites for Bone Regeneration 1 Marvin Lietzow
19:34 Oral Poster Alginate/silk fibroin-based hydrogels for vascularized tissue constructs 1 Dr. Raminder Singh
19:37 Oral Poster Diclofenac and moxifloxacin simultaneous delivery from intraocular lenses for endophthalmitis prophylaxis 1 Prof. Ana Serro
19:40 Oral Poster Optimization of a calcium phosphate based bone cement formulation 1 Prof. Dr. Rogério Cola?o
19:43 Oral Poster Nanostructured bone apatite-like thin films for bone prostheses 1 Dr. Gabriela Graziani
19:46 Oral Poster Low-dose BMP-2 enhances the bone-forming effects of an injectable, PLGA fiber-reinforced, brushite-forming cement in sheep lumbar osteopenia defects 1 Francesca Gunnella
19:49 Oral Poster The PLGA fiber component of brushite-forming calcium phosphate cement induces the osteogenic differentiation of human adipose tissue-derived stem cells 1 Francesca Gunnella
19:52 Oral Poster Surface functionalization of nanofibers by plasma processing in Ar/CO2/C2H4 discharge 0 Ph.D. Elizaveta Permyakova
19:55 Oral Poster Three-dimensional nanoscale X-ray computed tomography for the visualisation of cells grown on fibrous scaffolds 1 Dr. Juliana Martins de Souza e Silva

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Background and objectives

Despite major improvements in surgical procedures, bacterial infections remain a leading cause of titanium implant failure. Given the significant failure rates in treating implant associated infections, novel approaches are urgently needed to prevent the development of pathogenic biofilms on titanium implants. The specific objectives of this research are to generate nanostructured titanium surfaces that efficiently kill bacteria upon contact, which could be used to develop next generation, anti-infective implants. By studying how bacteria interact with, and respond to titanium dioxide nanostructure arrays, we aim to apply this understanding to design the most effective antibacterial implant surface.

 

Methods

Thermal oxidation was used to generate a library of titanium dioxide nanostructure arrays on grade 5 titanium surfaces. The antibacterial performance of each nanostructured surface was determined by metabolic indicator assays, bacterial viability techniques and confocal microscopy. Electron microscopy techniques (SEM, TEM and FIB-SEM) were used to visualise the interactions between bacteria and nanostructures and determine their effects on bacterial morphology. Quantitative proteomic approaches (TMT) were used to investigate the impact of nanostructures on bacterial cell physiology

 

Results


Significant reductions in viability were observed against Gram-positive (Staphylococcus aureus, Staphylococcus epidermidis) and Gram-negative (Escherichia coli, Klebsiella pneumoniae) bacteria. Combined imaging analysis confirmed that nanostructures can stretch and penetrate the bacterial cell envelope upon contact. Nanostructures also acted as physical barriers, effectively trapping cells and impeding their growth.

 

Conclusions

Titanium dioxide nanostructured surfaces showed a broad antimicrobial effect compared to flat titanium surfaces; the interplay between envelope stretching and cell trapping are proposed to have caused the reduction in bacterial viability.

Speaker:
Ph.D. Joshua Jenkins
University of Bristol
Additional Authors:
  • Dr. Angela Nobbs
    University of Bristol
  • Prof. Dr. Paul Verkade
    University of Bristol
  • Prof. Dr. Bo Su
    University of Bristol
  • Satishkumar Kulkarni
    Deutsches Elektronen-Synchrotron DESY
  • Dr.-Ing. Thomas F. Keller
    Deutsches Elektronen-Synchrotron DESY