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Poster

Durability analysis of the cryopreservation of DNA origami nanostructures

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Line-Up:
- Plenary Lecture Test Ploetz 1 Alexia Ploetz
- Poster Investigating Dynamic Biological Processes with High-Speed, High-Resolution Correlative AFM-Light Microscopy 1 Oilibhe Pabsch
- Poster Corrosion behaviour of potencially biodegradable ternary alloy Zn-Mg-Ca 1 Dipl.-Ing. Jan Pinc
- Poster Multi scale topography in titanium alloys by electron beam technique: influence on the tissue adhesion and bacterial contamination 1 Dr. Fernando Warchomicka
- Poster Cytotoxicity of gold nanorods and nanoribbons in context of biomedical applications 1 Marika Musielak
- Poster Preliminary studies of synthesis of gold nanoribbons using selected dicationic surfactants 1 Joanna Patalas
- Poster Diclofenac release from a silicon based hydrogel for contact lenses controlled by a layer-by-layer coating 1 Prof. Ana Serro
- Poster Synthesis, solubility and optical activity of chiral poly(2,4-disubstituted-2-oxazoline)s 1 Mengshi Yang
- Poster Influence of Cu2+-doping in β-TCP on the hydration kinetics of brushite cement investigated by Rietveld analysis and heat flow calorimetry 1 Karla Späth
- Poster Analytical investigation of the influence of micro- and nanoplastic particles in crop plants by electron microscopic imaging and spectroscopy 1 Dr. Jörg Wissler
- Poster Influence of fluoride corrosion attack on the metal-porcelain bond strength of TiCr20 compared to titanium 1 Christine Schille
- Poster Bacterial nanocellulose loaded with frankincense extract as natural wound dressing to treat local dermal inflammations 1 Berit Karl
- Poster Graded functionalization of polydopamine coated fiber mats as potential implant for tendon-bone-transition 1 Sarah Oehmichen
- Poster Enhancing the functionalities of bioactive glass-based scaffolds by novel polymeric coatings 1 Dr. Liliana Liverani
- Poster Durability analysis of the cryopreservation of DNA origami nanostructures 1 Yang Xin
- Poster Effects of staple age on DNA origami nanostructure assembly and stability 1 Charlotte Kielar
- Poster Dual-setting brushite-silica gel cements – Variations of the monomer precursors and tuning of the setting parameters 1 Ib Holzmeister
- Poster Novel QCM-D/microscopy set-up for real-time analysis of bacterial binding onto biomaterial-coated sensors 1 Dr. Zeqian Xu
- Poster Chairside plasma activation of encapsulated implants: how fast is it? 1 Miguel Angel Garcia Chame
- Poster Nanostructured high-pressure laminate surfaces to reduce transmission pathways of pathogens in public transportation 1 Christian Helbing
- Poster Biomineralization of Recombinant Spider Silk Films 1 Vanessa J. Neubauer
- Poster Modification of bacterial cellulose to enhance anti-inflammatory wound dressing properties 1 Uwe Beekmann
- Poster Absolute Characterization of Synthetic Macromolecules used in Biomaterials Science Applications 1 Mandy Grube
- Poster Biodegradable thermoplastic materials for bimodal imaging containing the mineral present in the human body 1 Zaneta Górecka
- Poster Nano-rough titanium surfaces created by wet alkaline etching and PVD and their influence on bacterial adhesion as well as protein adsorption 1 Michaela Schürmann
- Poster Effective strategies for the controlling and dealing with transmission pathway of pathogens in air traffi 1 Christian Helbing
- Poster Cytotoxicity evaluation of biodegradable Zn-4Cu alloy using L929, Saos-2 and TAg cell lines 1 Ping Li
- Poster Influence of pulsed bias voltage on the tribological and morphological properties of a-C coatings deposited by an anodic arc method 1 Dipl.-Ing. Ingo Erdmann

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DNA origami nanostructures as first presented by Rothemund have demonstrated great potential as functional platforms for various biomedical applications. Due to their broad applicability in technology and science, stability analysis of DNA origami structures is of high importance. While in the academic setting, experiments are typically performed using freshly prepared DNA origami samples, technological applications will require the storage of structurally intact DNA origami for extended periods of time. Among preservation processes, lyophilization has been discussed as a promising method for long term preservation of DNA origami structures at ambient temperatures. One drawback of this method is that it requires temperatures of down to -80 °C which may not be reached using typical lab equipment. In another work, freezing has been shown to reduce the lifetime of DNA molecules under tension.

Here, we thus investigate the durability of triangular DNA origami structures under repeated freezing and thawing using single-molecule atomic force microscopy. The DNA origami triangles maintain their structural integrity over many freezing and thawing cycles, up until a certain threshold after which the fraction of intact DNA origami is rapidly decreasing. We also evaluate the possibility to further increase this threshold by addition of cryoprotectants.

Speaker:
Yang Xin
Paderborn University
Additional Authors:
  • Charlotte Kielar
    Paderborn University
  • Xiaodan Xu
    Paderborn University
  • Siqi Zhu
    Paderborn University
  • Nelli Gorin
    Paderborn University
  • Prof. Dr. Guido Grundmeier
    Paderborn University
  • Dr. Adrian Keller
    Paderborn University