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Oral Poster

Parvovirus B19 virus-like particles as platform for the development of biomaterials.

Part of:
Line-Up:
18:40 Oral Poster Effect of surface processing on roughness, wettability and cytotoxicity of carbon fiber-reinforced polyetheretherketone (PEEK) composite fabricated by fused deposition modeling 1 Dr. Xingting Han
18:43 Oral Poster Characterisation of bactericidal titanium surfaces using electron microscopy 1 Ph.D. Joshua Jenkins
18:49 Oral Poster Parvovirus B19 virus-like particles as platform for the development of biomaterials. 1 Prof. Dr. Ismael Bustos-Jaimes
18:52 Oral Poster The Influence of Surface Nanopatterns with varying Periodicities on Protein adsorption 1 Yu Yang
18:55 Oral Poster Opaque, Translucent and Transparent Flower Petals 1 Takumi Arakawa
18:58 Oral Poster Impact of Surface Morphology and Net Charge of Spider Silk based Materials on Interactions with Human Blood Components 1 Sarah Lentz
19:01 Oral Poster Modulation of Bacteria on Titanium Surfaces via Nanotopography and Antimicrobial Peptides 1 Marcus Eales
19:04 Oral Poster Degradable hybrid polymers for tissue engineering 1 Johannes Schwaiger
19:07 Oral Poster Antibacterial functionalization of plasma-chemical oxidized titanium surfaces 1 Dr. Stefan Kranz
19:10 Oral Poster Thermal crosslinking of hydrophilic polymers using polyperoxides 1 Ph.D. Solomiia Borova
19:13 Oral Poster Poly(peptoid)s: Peptidomimetics as potential Biomaterials 1 Christine Schlutt
19:16 Oral Poster A Nanoscaled Hybrid Coating for Neuronal Electrodes: Nanoporous Silica NPs Embodied into Nanoporous Platinum. 1 Tim-Joshua Pinkvos
19:19 Oral Poster Drug Delivery from Nanoporous Titania Coatings for Dental Application 1 Saskia Zailskas
19:22 Oral Poster Waste to resource: Biopolymer from industrial wastewater 1 Prof. Regina Nogueira
19:25 Oral Poster Divide and Comprehend: Separating the Effects of Hydrophobicity / Hydrophilicity and Crystallinity on the Thermal Properties of Polyester Based Drug Delivery Systems 1 Karl Scheuer
19:28 Oral Poster Elastic Polymer-Coated Nano- and Macroporous Bioactive Glass-Composites for Bone Regeneration 1 Marvin Lietzow
19:34 Oral Poster Alginate/silk fibroin-based hydrogels for vascularized tissue constructs 1 Dr. Raminder Singh
19:37 Oral Poster Diclofenac and moxifloxacin simultaneous delivery from intraocular lenses for endophthalmitis prophylaxis 1 Prof. Ana Serro
19:40 Oral Poster Optimization of a calcium phosphate based bone cement formulation 1 Prof. Dr. Rogério Cola?o
19:43 Oral Poster Nanostructured bone apatite-like thin films for bone prostheses 1 Dr. Gabriela Graziani
19:46 Oral Poster Low-dose BMP-2 enhances the bone-forming effects of an injectable, PLGA fiber-reinforced, brushite-forming cement in sheep lumbar osteopenia defects 1 Francesca Gunnella
19:49 Oral Poster The PLGA fiber component of brushite-forming calcium phosphate cement induces the osteogenic differentiation of human adipose tissue-derived stem cells 1 Francesca Gunnella
19:52 Oral Poster Surface functionalization of nanofibers by plasma processing in Ar/CO2/C2H4 discharge 0 Ph.D. Elizaveta Permyakova
19:55 Oral Poster Three-dimensional nanoscale X-ray computed tomography for the visualisation of cells grown on fibrous scaffolds 1 Dr. Juliana Martins de Souza e Silva

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Virus-like particles (VLPs) are composed of the structural components of virions without its genetic material, producing non-infective particles that conserve the structural properties of virions, as immunogenicity and cellular-receptor recognition. Many VLPs are fabricated in eukaryotic cells, generating fully self-assembled particles. In contrast, other VLPs can be assembled in vitro from denatured proteins. Parvovirus B19 VLPs can be produced either in eukaryotic cells or in vitro from its denatured VP2 protein. The ability of these particles to self-assemble in vitro has led our group to assemble particles composed from chimeric forms of the same protein with excellent results. We have engineered VP2 to accommodate peptides and complete proteins in surface loops as well as at the N-terminus of this protein. We hypothesized that it would be possible to co-assemble more than one chimera of VP2, producing hybrid particles with more than one heterologous function.


Herein we present the construction of VLPs carrying both, lipase and GFP domains fused to the structural VP2 protein, by co-assembly of the chimeric proteins and VP2. The resulting species displaying lipolytic activity and fluorescence were purified by SEC and analyzed by DLS and AFM to confirm its structural properties. This material is the proof of concept for the in-vitro construction of VLPs displaying different functional domains through the co-assembly of chimeric forms of VP2. These particles may help to carry catalytic or regulatory activities to target cells by selecting suitable chimeras of VP2 that produce not only those activities, but also the precise tropism.

 

Speaker:
Prof. Dr. Ismael Bustos-Jaimes
National Autonomous University of Mexico
Additional Authors:
  • Dr. Maribel Cayetano-Cruz
    National Autonomous University of Mexico
  • Carla E. Segovia-Trinidad
    National Autonomous University of Mexico